| Laser Positioning | | Print | |
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Laser Positioning - Length and diameter measurement The natural complement to fast calcium measurements in muscle is a fast way to record the movement. This can be at the subcellular (sarcomere) level, cellular level or intact tissue level. For striated muscle we developed a laser diffraction method for real time measurement of sarcomere length measurements. By using a red 5 mW laser to penetrate the muscle one gets an accurate measurement based on an average of all myocytes that are penetrated by the light. Typically the entire cross section of the preparation. In addition to the average sarcomere length a Gaussian distribution of sarcomere length in the tissue can be recorded and stored. The distribution is very much linked to the health of the preparation and avoids the pitfalls of two dimensional mapping of sarcomere length in superficial layers of a preparation that may develop hypoxic cores during an experiment. At the cellular and tissue level we developed a linear camera based on a linear diode array chip. This camera provides a very fast measurement of sarcomere length, cell length or diameter of the tissue at 1 kHz. The technology is available for OEM. Among others the described technologies are used in the following SIH products: SARCSCR - manual laser based measurement of sarcomere length SARCCAM - linear camera & laser based measurement of sarcomere length at 1 kHz LINCAM - linear camera for dimensional analysis of cell length, tissue diameter |